Cisplatin assay

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August undefined, 2024

WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … WebAdjuvant cisplatin, 70 mg every three weeks, was given, for a total of 1680 mg. In February 1979, while he was receiving cisplatin, fever developed, accompanied by dark urine, a …

Quantitative analysis of cisplatin sensitivity of human ... - PubMed

WebMay 26, 2024 · Cisplatin is a well-known chemotherapeutic drug for solid tumors and leukemia with muscle toxicity, and long-term cisplatin medication can induce aberrant ROS accumulation in muscle cells and result in systemic muscle impairments ( Sakai et al., 2014; Conte et al., 2024 ). WebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer and incubated with anti-USP14 (ab70311; Abcam), anti-EGFR (ab52894; Abcam), or normal IgG (sc-2027; Santa Cruz ... the overman

The latest advances of cisplatin liposomal formulations: essentials …

WebAug 8, 2010 · Cisplatin is one of the most effective anticancer agents widely used in the treatment of solid tumors. It is generally considered as a cytotoxic drug which kills cancer cells by damaging DNA and inhibiting DNA synthesis. How cells respond to cisplatin-induced DNA damage plays a critical role in deci … WebApr 2, 2024 · The assay was optimized using Analysis of Variance (ANOVA) to identify potential experimental confounders and Z-factor (Z), … WebAssay preparation— Dissolve about 100 mg of Cisplatin, accurately weighed, in dimethylformamide in a 100-mL volumetric flask, dilute with dimethylformamide to volume, and mix. Chromatographic system (see … the overman culture

Application Note - Agilent Technologies

WebAug 6, 2015 · The chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, by conducting different omics data analyses, we demonstrated that cisplatin … WebSep 30, 2024 · Cisplatin (cis-diamminedichloridoplatinum (II), CDDP) is one of the most commonly used antineoplastic drugs worldwide. The platinum-based compound has been in clinical use for more than 40 years and is a cost-effective first-in-line treatment against several solid cancers including ovarian, head and neck and testicular cancer ( 1 ). shuri black panther bookWebSep 23, 2016 · The subsequent intracellular signaling pathway analysis indicated that pAkt and p62 expression levels correlated with alterations in the IC50 values for cisplatin in ovarian cancer, providing an... shuri black panther fanart

"WebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 … " - Cisplatin assay

Cisplatin assay

The changing 50% inhibitory concentration (IC50) of …

WebElectron microscopywas used to observe ultrastructure changes in cell. Results: The depletion of reduced glutathione caused by cisplatin and the inactivation of glutathione peroxidase played the vital role in the underlying mechanism. WebMar 1, 2024 · The developed and validated assay presented here is a highly sensitive LC-MS/MS method developed and validated for the quantitative determination of cisplatin following derivatization with diethyldithiocarbamate (DDTC) in order to detect platinum (Pt) of cisplatin, suitable for pharmacokinetic studies in rats and to further use it to study …

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WebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 inconsistencies. We showed that IC50 errors caused by the technical deficiencies of the MTT assay are large and not adjustable (range: 300-11,000%). WebCisplatin An anticancer agent WARNING: This product can expose you to chemicals including Cisplatin, which is [are] known to the State of California to cause cancer. For …

WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … WebOct 23, 2003 · Cisplatin is one of the most potent antitumor agents known, displaying clinical activity against a wide variety of solid tumors. Its cytotoxic mode of action is mediated by its interaction with ...

WebNov 1, 2005 · The assay was calibrated by addition of known amounts of cisplatin to drug-free plasma as described previously. Samples used for calibration contained cisplatin … WebFeb 15, 2024 · We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different "sensitizing ratio". Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance.

WebCisplatin is also sometimes used to treat head and neck cancer (including cancer of the mouth, lip, cheek, tongue, palate, throat, tonsils, and sinuses), lung cancer, cancer of the …

WebApr 11, 2024 · Cisplatin is a widely used chemotherapeutic agent for treatment of various cancers. However, treatment with cisplatin is associated with drug resistance and … shuri as black panther mcuWebNov 1, 2008 · Cisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human plasma and its applications to ... the overman group incAfter observing the enhanced cell-killing efficacy of the combination of CDDP and BV10 in various cancer cell lines and studying cell death processes, we further investigated the anti-cancer effect of this combination in the xenograft mouse models of lung, ovarian and cervical cancers. The typical dose of … See more The results of the effect of the combination of CDDP with BV10 on in vitro cell viability and clonogenic death are shown in Fig. 3. Figure 3A shows the … See more To demonstrate the DET mechanism of the combination, we made real-time measurements of the reaction between CDDP and BV10 … See more Figure 4A shows representative images of HeLa cells treated by CDDP with/without the combination with BV10 for 12 h, where apoptotic cells were … See more the overman group keller williamsWebApr 11, 2024 · The poster shows the final data from a phase 2 study evaluating the ability of the DRP ® companion diagnostic for cisplatin to prospectively identify patients with metastatic breast cancer that... the overman group morehead city ncWebOct 22, 2024 · Cisplatin is a commonly used anticancer drug, but nephrotoxicity is a dose-limiting adverse effect. Recent experimental and clinical observations have demonstrated that multiple injections of... shuri black panther hot toysWebMar 1, 2024 · Cisplatin (cis-diamminedicholroplatinum II, Fig. 1) is a platinum complex bound to two chloride atoms and two ammonia molecules which makes it highly soluble … the overman group kernersville nc the overmare studios