Erythroclear
WebRed blood cells (RBCs) were lysed using ACK lysing buffer. Cells were resuspended in RPMI supplemented with 10% FBS and 1% Pen/Strep. For human samples, cells were isolated from whole blood of healthy volunteers using ErythroClear RBC depletion kit (STEMCELL). Cells were resuspended in RPMI supplemented with 10% FBS and 1% … WebDec 6, 2014 · To address these issues, we have developed a simple and rapid immunomagnetic method (ErythroClear) for depleting RBCs in fresh and thawed CB. …
Erythroclear
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WebJun 29, 2024 · Single cells were sequenced using the 10× Genomics platform. Whole blood leukocytes (four males, four females) were isolated using a red blood cell depletion kit (Erythroclear Red Blood Cell Depletion Reagent Kit; STEMCELL Technologies, cat. no. 01738). Male and female samples were processed and sequenced in pairs to reduce … WebErythroClear™ is intended for the depletion of red blood cells (RBCs) from up to 16 small volume (50 - 100 µL) fresh or frozen cord blood samples at a time. ErythroClear™ reagent contains immunomagnetic particles that bind to Glycophorin AB+ cells, which are then selectively depleted using an ErythroClear™ magnet. ...
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WebShop Stemcell Technologies Inc ErythroClear™ Red Blood Cell Depletion Kit, For processing: 2 mL cord blood at Fishersci.com WebCells from patient blood were filtered over a size exclusion filter and red blood cells were removed using ErythroClear™ Red Blood Cell Depeltion from STEMCELL Technologies. Single cells were captured and libraries were constructed using the 10X Genomics, Chromium Box capture and library generation system. Library strategy: RNA-Seq: Library ...
WebErythroClear™ reagent contains immunomagnetic particles that bind to Glycophorin AB+ cells, which are then selectively depleted using an ErythroClear™ magnet. This …
WebThe ErythroClear™ Red Blood Cell Depletion Kit contains the ErythroClear™ Magnet and the ErythroClear™ Reagent intended for the depletion of red blood cells (RBCs) in small volumes (up to 100 μL) of fresh or frozen cord blood samples. Up to 16 cord blood samples can be processed at a time. Requires only 2 minutes per sample t series annual incomeWebJun 29, 2024 · Whole blood leukocytes were isolated using a red blood cell depletion kit (Erythroclear Red Blood Cell Depletion Reagent kit; STEMCELL Technologies, cat. no. 01738) Isolated whole blood leukocytes were encapsulated using microfluidics technology, and barcoded using a unique molecular identifier (UMI) t-series congratulations on scratch.mit.eduWebResults: •EasySep™ Direct Human Total Lymphocyte Isolation Kit was the most pure (93%) with no additional cell types isolated. •EasySep™ Direct Human PBMCIsolation Kit isolated 75% lymphocytes and a monocyte population (12.1%). •ErythroClear™ Red Blood Cell Depletion Kit left monocytes (2%), granulocytes (57%) and red blood cells (19%). ... t series channel youtubeWebFeb 24, 2024 · For human samples, cells were isolated from whole blood of healthy volunteers using ErythroClear RBC depletion kit (STEMCELL). Cells were resuspended in RPMI supplemented with 10% FBS and 1% penicillin/streptomycin (Pen/Strep) and treated with 100 ng/mL recombinant IFN-λ/IFN-α for 4 h. Single cells were sequenced using the … t series browning hi powerWebDec 6, 2014 · To address these issues, we have developed a simple and rapid immunomagnetic method (ErythroClear) for depleting RBCs in fresh and thawed CB. RBCs in small (≤100 µL) CB samples are labeled with... t-series congratulationsWebAug 15, 2024 · The granulocyte/RBC fraction was then enriched by dextran sedimentation followed by RBC lysis using hypotonic solution. Granulocytes were then washed with PBS. For WBC purification, whole blood samples were collected in heparinized tubes. WBCs were isolated with the Erythroclear RBC Depletion Reagent Kit (cat. no. 01738; STEMCELL … t series business parkWebErythroClear™ Red Blood Cell Depletion Kit (Catalog #01739). Alternatively, RBCs may be depleted using reagents that do not contain detergents or fixatives (e.g. Ammonium Chloride Solution; Catalog #07800). 2. Adjust the sample to a concentration of 1 x 10^6 cells/mL with the ALDEFLUOR™ Assay Buffer. t series facebook tan